Endogenous auxin and its manipulation influence in vitro shoot organogenesis of citrus epicotyl explants
نویسندگان
چکیده
منابع مشابه
In vitro shoot organogenesis from Eucalyptus sp. leaf explants
Background In vitro organogenesis is one of the key techniques associated with genetic transformation, as it determines the successful regeneration of transgenic plants after co-cultivation with bacteria. Therefore, the development of efficient regeneration protocols is the most critical step in developing genetic transformation. Protocols have been developed for several species of eucalyptus. ...
متن کاملCallus Induction and Shoot Regeneration from Epicotyl Explants of Ethnomedicinally Important Caesalpinia bonduc (L.) Roxb
An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...
متن کاملIndirect shoot organogenesis from leaf explants of Adhatoda vasica Nees
A novel protocol for indirect shoot organogenesis of Adhatoda vasica was developed using petiole explants derived from mature shrubby plants. Media with concentrations of cytokinins in combination with auxins were used to induce callus formation in two explants types: petiole and leaf segment. The frequency of callus formation from petiole and leaf segment explants on Murashige and Skoog (MS) b...
متن کاملIn Vitro Multiple Shoot Induction from Nodal Explants of Citrus Cultivars
Citrus cultivars were explored for multiple shoot induction and root regeneration in different media. The multiple root and shoot induction was found directly proportionate to the increase in the levels of benzyleaminopurine (BA) and naphthalene acetic acid (NAA) in the modified Murashige and Skoog medium. The study might be promising towards in vitro propagation of sanitated Citrus plant mater...
متن کاملIn Vitro Organogenesis in Some Citrus Species1
In vitro organogenesis of citrus was studied for the genotypes Citrus sinensis cv. ‘Natal’, C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP – 0.0; 0.5; 1.0; 1.5, or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. auranti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Horticulture Research
سال: 2017
ISSN: 2052-7276
DOI: 10.1038/hortres.2017.71